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快速细胞毒性(发光)测试盒 EnzyLight™ Cytotoxicity Assay Kit

简要描述:北京群晓科苑是博世BioAssay Systems授权代理商。 博世BioAssay Systems 生物产品主要包括:1、血液尿液化学;2、酶活性测定;3、能量代谢;4、氧化应激;5、高通量HTS试剂;6、阴阳离子检测。

  • 产品型号:ECTX-100
  • 厂商性质:代理商
  • 更新时间:2017-10-12
  • 访  问  量:710

产品介绍

快速细胞毒性(发光)测试盒 

EnzyLight™ Cytotoxicity Assay Kit

 

 

应用分类  高效药物筛选

 

测定方法    快速荧光素/荧光素酶发光法检测细胞活性、细胞增殖、细胞毒性,用于抗癌药物的高通量筛选。

 

Description    Adenosine 5-triphosphate (ATP) is the chemical energy for cellular metabolism and is often referred to as “energy currency" of the cell. ATP is produced only in living cells during photosynthesis and cellular respiration and consumed in cellular processes including biosynthetic reactions, motility and cell division. It is a key indicator of cellular activity and has been utilized as a measure of cell viability and cytotoxicity in research and drug discovery.our EnzyLightTM Cytotoxicity Assay Kit provides a rapid method to measure intracellular ATP, cell viability and cytotoxicity. The single working reagent lyses cells to release ATP, which, in the presence of luciferase, immediay reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration and hence number of living cells.This non-radioactive, homogeneous cell-based assay can be conveniently performed in microplates. The reagent is compatible with all culture media and liquid handling systems for high-throughput screening applications in 96-well and 384-well plates.

 

Key features     Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay).  Sensitive and accurate. As low as 50 cells can be quantified.Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.Robust and amenable to HTS: Z’ factors of 0.6 to 0.7 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

 

Applications    Cell proliferation: effects of cytokines, growth factor, nutrients.Cytotoxicity and apoptosis:evaluation of toxic compounds, anti-cancer antibodies, toxins, environmental pollutants etc. Drug discovery: high-throughput screening for anticancer drugs.

 

Storage conditions: store all reagents at -20°C. Shelf life of at least 6 months.

 

 

产品信息

 

  货号

产品名称

规格

单价(元)

ECTX-048

快速细胞毒性(发光)测试盒

48T

998.45

ECTX-100

快速细胞毒性(发光)测试盒

100T

1550.4

 

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