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荧光素酶报告基因测试盒 SuperLight™ Luciferase Reporter Gene

简要描述:北京群晓科苑是博世BioAssay Systems授权代理商。 博世BioAssay Systems 生物产品主要包括:1、血液尿液化学;2、酶活性测定;3、能量代谢;4、氧化应激;5、高通量HTS试剂;6、阴阳离子检测。

  • 产品型号:SLLU-048
  • 厂商性质:代理商
  • 更新时间:2017-10-12
  • 访  问  量:658

产品介绍

荧光素酶报告基因测试盒 |

SuperLight™ Luciferase Reporter Gene Assay Kit

 

 

应用分类  高效药物筛选

 

测定方法   快速(2分钟内)生物发光法测定荧光素酶报告基因在细胞中的表达。

 

Description    The SuperLightTM Luciferase Reporter Gene Assay is based on the quantitation of luciferase expression in mammalian, yeast or E. coil cells, using luciferin and ATP as substrates. The reaction results in light production which can be conveniently measured on a luminometer Reaction scheme:This bioluminescent reporter gene assay is extremely sensitive and is especially suitable for quantifying luciferase expression in recombinant cells. This ultra-sensitive, homogeneous cell-based assay only requires adding a single reagent to the cells and measuring the light intensity after a short incubation step (2 minutes). Assays can be performed in tubes, cuvettes or multi-well plates. All kit components are compatible with culture media and with all liquid handling systems. With an extended luminescence emission kinetics (half-life 40 min), the SuperLightTM luciferase assays are especially suitable for high-throughput screening in 96-well, 384-well and 1536-well plates. In addition, the reagent provided in the kits has been formulated for maximum sensitivity, reproducibility and long shelf-life. Applications for this kiinclude gene t regulation studies and high-throughput screening of gene modulators.

 

Key features    High sensitivity and wide detection range: detection of as little of 2 fg luciferase and as few as 4 cells. Plus, the emitted light is linear over seven orders of magnitude.Compatible with routine laboratory and HTS formats: assays can be performed in tubes or microplates, on LJL Analyst, Berthold Luminometer, Top-Count, MicroBeta counters, chemiluminescent image plate readers (CLIPR/LeadSeeker). Assay reagents compatible with all liquid handling systems.Fast and convenient: homogeneous “mix-and-measure” assay allows detection of luciferase levels within 10 minutes. The optimally combined reagent system allows a single addition step, and simultaneous cell lysis and detection.Robust and amenable to HTS: Z’ factors of 0.6 to 0.8 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.

 

Applications    Gene Regulation: gene expression level, characterization of promoter activity, modulation of gene expression by receptors, transcription factors and small molecules.Drug Discovery: high-throughput screen for gene modulators.

 

Storage conditions.  Store the Reagent in the provided amber tube at -20°C and the Assay Buffer at 2-8°C.  Shelf life: 12 month.

 

 

产品信息

 

  货号

产品名称

规格

单价(元)

SLLU-048

荧光素酶报告基因测试盒

48T

1749.9

SLLU-200

荧光素酶报告基因测试盒

200T

1583.65

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