EnzyChrom™ Glycogen Assay Kit
应用分类 血液尿液化学, 代谢产物分析
Description GLYCOGEN is a branched polysaccharide of glucose units linked by α-1,4 glycosidic bonds and α-1,6 glycosidic bonds. It is stored primarily in the liver and muscle, and forms an energy reserve that can be quickly mobilized to meet a sudden need for glucose. The most common glycogen metabolism disorder is found in diabetes, in which, due to abnormal amounts of insulin, liver glycogen can be abnormally accumulated or depleted. Genetic glycogen storage diseases have been associated with various inborn errors of metabolism caused by deficiencies of enzymes necessary for glycogen synthesis or breakdown.Simple, direct and automation-ready procedures for measuring glycogen concentrations find wide applications in research and drug discovery. our glycogen assay uses a–amylase/amyloglucosidase hydrolysis reagent that rapidly breaks down glycogen into glucose, which is determined with our proprietary colorimetric glucose assay reagent. This simple convenient assay is carried out at room temperature and takes 45 min.
Key features Use as little as 20 mL samples. Linear detection range 0.9 to 50 mg/mL (9 to 500 ng) glycogen.
Storage conditions. The kit is shipped on dry ice. Store Standard and Detection Reagent at 4°C and other reagents at -20°C. Shelf life of three months after receipt.