insphero公司联合苏黎世大学的骨科研究实验室、化学和生物化学研究所,应用insphero 3D细胞悬滴培养板,构建3D骨肉瘤模型。
文章“An in vitro osteosarcoma 3D microtissue model for drug development”于2014年11月10号发表于 Journal of Biotechnology上
(链接地址:http://www.ncbi.nlm.nih.gov/pubmed/25234575)
作者介绍:
Markus Rimanna, 1, Sandra Laternsera, 1, Ana Gvozdenovicb, Roman Muffb, Bruno Fuchsb, Jens M. Kelmc, Ursula Graf-Hausnera, ,
a Institute of Chemistry and Biological Chemistry (ICBC), Zurich University of Applied Sciences, Waedenswil, Switzerland
b Laboratory for Orthopedic Research, Department of Orthopedics, University of Zurich, Switzerland
c InSphero AG, Schlieren, Switzerland
文章亮点:
1.Microtissues (3D) from three different osteosarcoma cell lines were developed.
取三种不同的骨肉瘤细胞系(SaOS-2、HOS和MG-63细胞),采用insphero 3D细胞悬滴培养板构建3D骨肉瘤微组织
2. Microtissues from patient-derived osteosarcoma were produced.
骨肉瘤细胞系(SaOS-2、HOS和MG-63细胞),均取自成骨肉瘤和软骨肉瘤病人
3.Inherent osteosarcoma tissue heterogeneity was shown with different microtissues.
不同的微组织,会显示出骨肉瘤固有的组织异质性
4.Five drug responses were analysed in 3D showing differences compared to 2D.
用五种药物(阿霉素、顺铂、牛磺罗定、培美曲塞、紫杉醇)对3D骨肉瘤微组织用药,实验结果是不同于常规2D细胞培养的
总结:
The 3D microtissues reflect the tissue heterogeneity of OS and are potential suitable tools for drug development towards personalised medicine
3D微组织能够反映出骨肉瘤(OS)固有的组织异质性,它将成为药物开发以及走向个人化医疗过程中的有潜力工具。